A warm welcome to DreamGene.

Our organization brings almost 20 years of experience in Molecular Diagnostics and Life Science on Polish and European markets. Our goal is to provide our Customers with the highest quality products for DNA analysis as well as professional advice and support.

We are an exclusive distributor of premium FISH probes from USA based company – CYTOTEST

These unique FISH probes, feature premium quality of hybridization / fluorescence, resulting in unrivalled ease of signal counting and interpretation. They are CE Marked according to IVD Directive, ready to use (suspended in hybridization buffer) and conveniently packed in 10 test (100 ul) vials. Finally we can offer highly competitive pricing comparing to other vendors on the market.

Currently we offer more than 300 premium quality, ready FISH probes.

Additionally, CytoTest is able to design and manufacture any custom probes, according to your needs.

Apart from the probes we offer all necessary FISH accessories and equipment.

While maintaining the highest product quality, we offer very competitive prices comparing to other vendors on the market.

For any additional information please visit the manufacturer’s website:

www.cytotest.com

or look through the electronic version of CytoTest FISH probes catalogue.

https://www.cytotest.com/uploadfiles/file/2021-11/CytoTest%20Catalog_V2021_12_14.pdf

We cordially invite you to collaborate.

With best regards,

Tomasz Czerski – founder / owner

Fluorescence in situ Hybridization (FISH)

In situ hybridization (ISH) is a powerful technique designed to detect presence or absence, location, integrity and amount of DNA or RNA sequences in tissues, cells or on chromosomes. ISH is based on the detection of specific sequences by pairing of bases (hybridization) on complementary single strands of nucleic acid. Here, one of the strands is a labeled sequence fragment (probe) that binds only to those parts of the genome with highly or completely complementary sequence, and the other strand is present in the sample material that is to be analyzed. Accordingly, in situ hybridization starts with preparing the sample to be analyzed and with preparing the probe. The typically double-stranded DNA in the sample has to be melted (denatured) into single strands, and the probe has to be labeled to enable detection.

The basic steps for ISH were developed in the 1960s using radioactive isotopes for probe detection. Starting in the early 1980s, protocols using fluorescent dyes for probe visualization have been developed. Because these labels are safer, more stable and easier to handle, the vast majority of in situ hybridization today is done using fluorescence ISH (FISH) protocols. One other important advantage of FISH is that it allows – by using several different fluorescent dyes – the simultaneous detection of different target structures within a single sample.

FISH has become a central cytogenetic tool. Probe fragments can be derived from various sources, by recombinant methods or by PCR. Today, accurately mapped bacterial artificial chromosomes (BAC) are the most frequently used probe type. Designing or selecting the right probe for FISH often requires a balance between sensitivity and specificity, i.e. a probe must be large enough to assure specific binding and to carry a sufficient amount of label for detection. On the other hand, with increasing length of the probe the possibility of hybridization to additional target sequences increases as well. In addition, if a probe is too long it may result in a large or diffuse signal.

Probe labeling can be done by linking fluorescent molecules directly to the probe or by tagging probe with antigenic or chemical linkers that will later bind to fluorophores. Depending on which protocol is used, the process can take anywhere from a few hours to a few days. It is called “in situ” to stress the fact that detection of target nucleic acid occurs not on isolated molecules in test tubes, but directly on fresh or fixed cells or tissues.

With FISH, chromosomal rearrangements or aberrations of as small as a few megabase pairs (million base pairs) can be made visible on metaphase (condensed) chromosomes. The resolution goes down to about 1Mb for interphase (spread out) chromosomes. Even greater resolution can be achieved with recently developed methods encompassing artificial stretching of chromatin fibers.

FISH methods cannot detect base-level mutations, e.g. the point mutations underlying some heritable disorders. However, FISH techniques have made landmark contributions in moving the genetic and physical maps of the human genome closer together.

In research, FISH is used to identify the position of genes, determine the number and integrity of human chromosomes, for karyotyping and chromosome painting. In the clinical laboratory, FISH has become a standard tool for detecting chromosomal aberrations, particularly in prenatal and cancer diagnosis. FISH probes and protocols for many genes have been analytically and clinically validated, and many more are being developed and tested.

DreamGene Tomasz Czerski

20-810 Lublin ul. Sempołowskiej 26

NIP PL 712-232-13-12

REGON 364199577

The CytoHYB CT500 denaturation and hybridization instrument is a programmable system for automated processing in slide-based FISH procedures.  The machine is equipped with several distinct advantageous features, including convenient touchscreen programming, integral USB port, unparalleled heating speed and accuracy, minimal temperature variation, and higher humidity range. Moreover, a unique design with liquid grooves allows for convenient humidification and stable humidity control in the chamber interior. In addition, the unit is easy to clean, and there is no need for disposable foam strips, or for any other accessory consumables that have to be frequently replaced.

Accepting a broad range of sample types, and equipped with slide guides that keep slides in place and allow for one-hand placement and removal, the low cost unit drastically reduces hands-on time without compromising precision and reproducibility. The product is suitable for a wide variety of denaturation and hybridization experimental strategies, with four distinct modes of operation: Denaturation/hybridization, Hybridization, Custom, and in situ PCR processes. The integrated heated water tank and secure-sealed heated lid provide experimental consistency to the system, which can process up to 12 slides simultaneously.

Available for operation with both 110-120V or 220-240V power supply.

DreamGene Tomasz Czerski

Biuro w Lublinie:

20-810 Lublin ul. Sempołowskiej 26

Biuro w Warszawie:

02-640 Warszawa ul. Woronicza 33/19

Tomasz Czerski

+48 601951254

fax + 48 22 350 68 21

tczerski75@gmail.com

Gregor Majewski – Business Development Manager D/A/Ch

gregorm2014@gmail.com